Microbial identification

Identification and typing of bacteria or fungi can be very costly and time consuming, because of growth time of the microorganisms and lab work. ProBase Pharma offers microbial identification services using established as well as innovative techniques under GMP conditions. Our methods are much faster and more reliable than currently used phenotypic methods. ProBase Pharma services are suitable for fast screening of even the most difficult samples.

Bacterial identification

MicroSEQ® identification

DNA-isolation, PCR, sequencing and reporting will be performed using MicroSEQ® validated protocols, kits and database. Approximately 500 base pairs of the 16S rRNA gene will be amplified and sequenced on both strands. Data is aligned using the MicroSEQ® validated software and reviewed manually by our technicians. A full report including the homology search to the MicroSEQ® validated database is prepared and reviewed by the QA department. After approval a QA signed report containing results and sequence data (fasta/.seq files) will be mailed to the client within 5 working days for the standard service or within one working day after we have received your sample(s) for our fast service!

MALDI-TOF identification

MALDI-TOF identification is achieved by obtaining spectra using the MALDI-TOF technology (Matrix Assisted Laser Desorption Ionization Time-of-Flight) and analyzing the spectra with the VITEK® MS database. The peaks from these spectra are compared to the characteristic pattern for a species, genus, of family of micro-organism, thus resulting in an identification.

Fungal identification

MicroSEQ® identification

DNA-isolation, PCR, sequencing and reporting will be performed using MicroSEQ® validated protocols, kits and database under GMP conditions. For fungal samples, the D2-LSU gene is amplified and sequenced on both strands using validated MicroSEQ® kits and protocols. The length of the consensus sequence varies and is approximately 200-300 bp long. Data is aligned using the MicroSEQ® validated software and reviewed manually by our technicians. A full report including the homology search to the MicroSEQ® validated database is prepared and reviewed by the QA department. After approval a QA signed report containing results and sequence data (fasta/.seq files) will be mailed to the client within 5 working days for the standard service or within one working day after we have received your sample(s) for our fast service!

In addition to our microbial identification services we also offer services like pure culturing and/or testing of more, other genes can be provided. Please contact Richard de Winter (directly +31 (0)6 20634893) or one of our account managers for more information at +31 (0)71 523 3917.